Table 1. Information of primers utilized for real-time PCR analysis

Gene	Sequence (5’ → 3’)	T_m(°C)	PCR product (bps)
Srd5a1 (NM_175283.3)	(F) GAGGCAGCATCATCAGTGGTAC (R) CACCGGGAAGACAGACACTCA	60	134
Cyp19a1 (NM_007810.4)	(F) TGTGTTGACCCTCATGAGACA (R) CTTGACGGATCGTTCATACTTTC	55	190
Ar (NM_013476.4)	(F) TGTCAAAAGTGAAATGGGACC (R) TGGTACTGTCCAAACGCATGT	55	74
Esr1 (AB560752.1)	(F) GCCTCTGGCTACCATTATGG (R) CATCTCTCTGACGCTTGTGC	55	244
Esr2 (U81451.1)	(F) GTCAGGGTGGTTGGGTTGTGG (R) ATCGCGTCACTTTCCTTTTCC	58	203
Gapdh (NM_008084.3)	(F) CCCCTGGCCAAGGTCATCCATGACAACTTT (R) GGCCATGAGGTCCACCACCCTGTTGCTGTA	60	513

Srd5a1, 3-oxo-5α-steroid 4-dehydrogenases type 1; Cyp19a1, cytochrome P450 aromatase; Ar, androgen receptor; Esr1, estrogen receptor alpha; Esr2, estrogen receptor beta; Gapdh, glyceraldehyde-3-phosphate dehydrogenase.