Table 1. Bioactivities of constitutive activating eel LH/CGR mutants
| Eel LH/CG receptors | cAMP responses |
| Basal1 (nM/104 cells) | Log (EC50) (ng/mL) | Rmax2 (nM/104 cells) |
| Eel LH/CGR-wt | 3.8±1.0 | 58.66 (48.1 to 75.3)3 | 64.5±1.7 |
| Eel LH/CGR-M410T | 5.2±0.9 (1.4-fold) | 21.7 (16.8 to 30.8) | 42.4±1.2 |
| Eel LH/CGR-L469R | 33.2±0.7 (8.7-fold) | 708 (373.4 to 6,965) | 67.9±3.4 |
| Eel LH/CGR-D590Y | 15.3±1.5 (4.0-fold) | 31.1 (22.5 to 50.2) | 64.8±2.2 |
Values are the means±SEM of triplicate experiments. The log (EC50) values were determined from the concentration-response curves obtained from the in vitro bioassays.
Basal cAMP level average without agonist treatment.
Rmax average cAMP level/104 cells.
Geometric mean (95% confidence limit) of at least three experiments.
LH, luteinizing hormone; CGR, choriogonadotropin receptor; cAMP, cyclic adenosine monophosphate; EC50, effective concentration.