Table 2. Primers used for quantitative real-time PCR (qRT-PCR) for reproduction and/or salinity related genes of eel pituitary cell
Gene | | Primer sequence | Product size |
β-actin | F | GAGACCTTCAACACCCCAG | 95 |
R | CCAGAGTCCATCACAATACCAG |
LHβ | F | GATCCCCATGTGACTTTCCC | 72 |
R | GCAGTCAGACGTGTCCATG |
FSHβ | F | CTCGCCAACATCTCCATCTC | 99 |
R | AGAATCCTGGGTGAAGCAC |
pgrl | F | GGGTAGTGACAGTGAGATTTGG | 116 |
R | CAGCCCAAGACAAATTTTCGG |
PRL | F | GATTCACAAGATTGGCTCCTC | 143 |
R | TTGTCGATTTTGTGGGAGTC |
drd4 | F | CGGATTTGGGACTGTACTTCTG | 92 |
R | TCGCTTGAGTGATATACGCAC |
NPBWR2 | F | AGTTGTATGCCTTCGGTTCC | 126 |
R | TCAATCCCACGGCACATATG |
rxfp3-2b | F | GTAATCTGTCCGCAACCAAAAG | 102 |
R | GACTGACAATAAAGTTCACGGTG |
LHβ, luteinizing hormone; FSHβ, follicle stimulating hormone; pgrl, progesterone receptor-like; PRL, prolactin; drd4, dopamine receptor D4; NPBWR2, neuropeptide B/W receptor 2; rxfp3-2b, relaxin family peptide receptor 3-2b.