Table 2. PCR conditions used in this study

Gene	Temperature & time	Number of cycles
	denature / annealing / extension
ATX	95°C, 35 sec / 54C, 35 sec / 72°C, 45 sec	41
GAPDH	95°C, 60 sec / 55C, 35 sec / 72C, 60 sec	28

ATX, autotaxin; GAPDH, glyceraldehydes-3-phosphate dehydrogenase.