Table 1. Bioactivity of eLH/CG receptors in cells expressing eLH/CGR-wt and eLH/CGR-t656 mutant

eLH/CG receptors	cAMP responses
	Basal1 (nM / 104 cells)	Log (EC50) (ng/mL)	Rmax2 (nM / 104 cells)
CHO-K1 cells eLH/CGR-wt	4.1±1.2	18.7 (14.1 to 27.8)3	51.7±1.7 (1-fold)
CHO-K1 cells eLH/CGR-t656	3.9±0.7	25.9 (19.2 to 39.7)	29.9±1.1 (0.57-fold)
PA CHO cells eLH/CGR-wt	6.2±1.5	27.6 (19.1 to 49.7)	53.0±2.4 (1-fold)
PA CHO cells eLH/CGR-t656	8.7±1.6	15.9 (10.7 to 28.4)	57.6±2.4 (1.08-fold)

Basal cAMP level average without agonist treatment.
Rmax average cAMP level/10⁴ cells.
Geometric mean (95% confidence limit) of at least three experiment. eLH/CGR, equine luteinizing hormone/chorionic gonadotropin receptor; CHO, Chinese hamster ovary.