Table 1. Bioactivity of rec-eCGβ/α mutants in cells expressing eLH/CG receptor

rec-eCG	cAMP responses
	Basal1) (nM /104 cells)	EC502) (ng/mL)	Rmax3) (nM /104 cells)
rec-eCGβ/α-wt	9.5±1.4	31.6 (1.0-fold) (26.4 to 39.4)4)	141.9±3.0 (1.0-fold)
rec-eCGβ/α∆56	5.4±0.7	65.8 (2.1-fold) (57.9 to 76.3)	80.2±1.3 (0.56-fold)
rec-eCGβ-D/α	6.5±1.1	176.8 (5.6-fold) (150.4 to 214.4)	114.3±2.9 (0.80-fold)
rec-eCGβ-D/α∆56	5.1±1.0	106.9 (3.4-fold) (90.6 to 130.2)	92.9±2.2 (0.65-fold)

Basal cAMP level average without agonist treatment.
Half maximal effective concentration.
Rmax average cAMP level/10⁴ cells.
95% Confidence intervals.