| 1 | Eel LHR-wt forward | 5'-ATGAATTCATGTCCAATCTGCTCTTGTGGACGATG-3' EcoRI site |
| 2 | Eel LHR-wt reverse | 5'-CCTCGAGTTATTTAGGACCTCTGTTGAGAAT-3' XhoI site |
| 3 | M410T forward | 5'-TCTCCCGCTTCCTCACGTGCAACCTGGCCTT-3' |
| 4 | M410T reverse | 5'-AAGGCCAGGTTGCACGTGAGGAAGCGGGAGA-3' |
| 5 | L469R forward | 5'-TGTCCGTCTACACCCGGACCGTCATCACCCT -3' |
| 6 | L469R reverse | 5'-AGGGTGATGACGGTCCGGGTGTAGACGGACA-3' |
| 7 | D590Y forward | 5'-TGCTCATATTCACCTACTTCCTGTGCATGGC-3' |
| 8 | D590Y reverse | 5'-GCCATGCACAGGAAGTAGGTGAATATGAGCA -3' |
| 9 | D417N forward | 5'-CTGGCCTTCGCCAACCTCTGCATGGGC -3' |
| 10 | D417N reverse | 5'-GCCCATGCAGAGGTTGGCGAAGGCCAG-3' |
| 11 | Y558F forward | 5'-GTCTGCGTCTGCTTCGGCCGCATCTAC-3' |
| 12 | Y558F reverse | 5'-GTAGATGCGGCCGAAGCAGACGCAGAC-3' |