Table 1. Characteristics of CCDC110 mAbs

mAb Clone name Isotype Immunoblotting reactivity against
M1 5A10C12G3 IgG1 E (±), O (+++)
M2 4D9A12D4 IgG2b E (+), O (+++)
M3 4D9A12B10 IgG2b E (+), O (+++)
M5 1A6H10 IgG1/IgG2b E (±), O (+++) IP (++)*
M6 3A1E12 IgG1 E (±), O (+++)
M7 2H11H7 IgG1 E (±), O (++) IP (+)**
M8 A-4-3 IgG1 E (+), O (+++)
M11 B-10 IgG1 E (+), O (+++)
M12 C-12-12 IgG1 E (+), O (+++)
M5 clone can immunoprecipitate both endogenous and ectopically overexpressed protein.
M7 clone immunoprecipitate ectopically overexpressed protein.
The detection of endogenous protein was assessed only in immunoprecipitated samples.
CCDC110, coiled-coil domain containing 110; E, endogenous; O, overexpressed; IP, immunoprecipitated.