Table 2 Comparison of blood and plasma factors during 120 hrs after hyper osmoregulation of salinity in river puffer, Takifugu obscurus, tiger puffer, T. rubripes, hybrids (river puffer ♀× tiger puffer ♂), and hybrid triploids*

Items	Elapsed time (hrs)	Experiment groups
Items	Elapsed time (hrs)	River puffer	Hybrids	Hybrid triploids	Tiger puffer
Hematocrit (%)	0	24.4±4.9^a	24.3±5.9^a	25.4±4.1^b	28.4±5.1^a
	24	32.5±3.2^ab	41.8±8.6^b	24.1±7.2^a	35.5±3.9^ab
	48	38.9±7.4^b	40.0±6.5^b	43.2±5.5^c	38.0±8.1^b
	72	31.0±5.6^ab	28.6±9.0^a	38.4±6.4^b	31.4±6.0^ab
	96	31.8±7.4^a	35.3±7.2^ab	40.7±9.7^bc	31.0±5.1^ab
	120	25.8±3.0^a	29.1±3.3^ab	36.6±3.4^bc	24.8±2.4^a
Hemoglobin (g/dL)	0	9.8±0.3^a	10.1±0.6^a	10.1±0.7^a	10.8±1.0^a
	24	12.8±0.8^ab	14.8±0.4^ab	11.1±3.0^a	12.1±0.9^ab
	48	14.5±2.3^b	17.5±4.1^b	15.8±0.6^b	15.5±3.1^b
	72	14.3±2.3^b	14.9±3.4^ab	14.5±1.3^b	14.8±2.8^b
	96	11.0±1.3^ab	13.0±1.3^ab	14.4±0.9^b	12.4±1.5^ab
	120	12.4±0.7^ab	10.6±3.9^a	14.7±0.1^b	11.0±1.1^ab
Red blood cell (×10⁶cell/µL)	0	2.7±0.7^a	2.8±0.5^a	2.6±0.5^ab	2.9±1.1^a
	24	3.6±0.1^ab	4.3±0.3^b	2.2±0.6^a	3.8±0.5^ab
	48	5.9±1.0^c	5.1±2.9^c	5.9±1.4^c	6.1±0.9^c
	72	3.3±0.4^ab	3.8±0.9^b	4.2±0.6^abc	3.5±0.7^ab
	96	2.3±0.6^a	4.0±1.3^b	5.7±1.5^c	2.8±0.4^a
	120	2.5±1.6^a	4.4±0.7^b	4.5±1.3^bc	2.5±0.6^a
Na⁺ (mEq/L)	0	163.5±2.1^ab	162.5±3.5^a	166.0±5.7^ab	164.8±3.5^ab
	24	171.7±3.5^c	173.3±4.9^ab	170.0±5.3^bc	173.9±4.0^c
	48	168.0±4.6^bc	168.7±3.8^ab	171.0±6.2^bc	169.1±3.1^bc
	72	166.3±2.5^abc	165.3±4.6^ab	166.7±4.9^ab	167.5±3.4^abc
	96	161.0±2.0^a	169.3±2.9^ab	164.0±3.6^a	161.5±1.8^a
	120	166.0±2.6^abc	166.3±3.5^b	168.3±2.3^b	165.7±3.7^abc
K⁺ (mEq/L)	0	3.6±0.2^b	3.7±0.4^b	3.9±0.2^b	3.8±0.2^b
	24	3.3±0.3^ab	4.2±1.4^bc	4.1±1.3^bc	3.5±0.1^ab
	48	3.3±0.2^ab	3.1±1.0^a	4.0±1.4^bc	3.4±0.3^ab
	72	3.2±0.1^ab	2.9±0.2^a	2.9±0.2^a	3.3±0.2^ab
	96	3.1±0.4^ab	3.1±0.3^a	3.1±0.3^a	3.2±0.3^ab
	120	3.0±0.1^a	3.2±0.4^a	3.3±0.4^a	2.8±0.1^a
Cl⁻ (mEq/L)	0	135.5±0.7^a	137.5±3.5^a	139.0±4.2^b	131.4±1.9^a
	24	141.0±2.0^b	145.0±3.5^bc	135.0±2.8^a	143.8±3.8^b
	48	139.7±5.1^ab	139.3±2.3^ab	142.0±5.0^c	139.1±3.1^ab
	72	138.3±1.2^ab	143.3±5.7^b	137.7±6.0^ab	137.7±2.2^ab
	96	136.0±3.0^ab	144.3±3.5^b	137.3±3.1^ab	136.5±1.8^ab
	120	138.7±4.2^ab	137.7±2.5^a	140.0±1.0^bc	135.7±3.8^ab
Aspartate aminotransferase (IU/L)	0	04.0±2.8^abb	5.1±0.1^ab	5.4±0.9^ab	05.1±1.4^abb
	24	05.5±0.7^bab	3.3±2.1^ab	3.3±1.5^ab	05.5±2.1^ccc
	48	09.0±5.7^caa	8.0±1.4^bb	4.7±3.1^ab	011.5±4.9^bcc
	72	05.5±2.1^abb	2.0±1.4^ab	4.0±2.6^ab	09.7±4.8^abc
	96	03.5±4.9^acb	1.5±0.7^ab	2.5±2.1^ab	04.9±3.1^abb
	120	02.3±0.6^abb	4.3±2.3^ab	6.0±3.6^ab	02.1±0.4^abb
Alanine aminotransferase (IU/L)	0	01.7±1.2^abb	2.2±0.2^ab	1.5±0.6^ab	01.8±0.7^abb
	24	02.0±0.0^abb	1.7±1.2^ab	1.3±0.6^ab	02.1±0.1^abb
	48	02.5±0.7^abb	2.5±0.7^ab	2.3±1.5^ab	03.2±0.6^bbb
	72	01.7±1.2^abb	1.3±0.6^ab	2.7±1.2^ab	01.9±0.8^abb
	96	01.7±0.2^abb	1.3±0.6^ab	2.0±1.4^ab	01.4±0.3^abb
	120	01.3±0.6^abb	2.0±0.0^ab	2.7±1.5^ab	01.3±0.6^abb