Table 1. Primer sequences for real time PCR
Gene | Primer sequence (5′ → 3′) | Product size (bp) | Annealing temp. | Accession number |
uPA | F: TGCATCGAACTGTGGCTGTC | 146 | 60°C | NM_213945 |
R: GACCGTTCCCCTCAAAGCAG |
tPA | F: CAGCCGCTGCACTTCAAAAC | 222 | 60°C | NM_214054 |
R: GTGTACACACCCGGAACGTC |
uPAR | F: GCACACATGGGAAGGAGGTG | 214 | 60°C | XM 003127198 |
R: CGAGGCACAGGAAGCACATT |
PAI-1 | F: CACCGCCTCTTCCACAAGTC | 149 | 60°C | NM_213910 |
R: ATGCTCAGAGTGTTGCCGTG |
GAPDH | F: CATGGTTCACGCCCATCACA | 170 | 60°C | NM_001206359 |
R: TCCCGCCAACATCAAATGGG |
uPA, Urokinase-type plasminogen activator; tPA, Tissue-type plasminogen activator; uPAR, Urokinase-type plasminogen activator receptor; PAI-1, Type-1 plasminogen activator inhibitor; GAPDH, Glyceraldehyde-3-phosphate dehydrogenase.