Table 1. Primer sequences and condition using real-time PCR
Gene | Primer sequence (5′ → 3′) | Product size (bp) | Annealing temp. | Accession number |
FADS1 | F: CGTGATTGACCGGAAGGTGT | 110 | 60°C | NM 001113041.1 |
R: CCACAAAGGGATCCGTAGCA |
FADS2 | F: CCGGTTGAGTACGGCAAGAA | 217 | 60°C | NM_001171750.1 |
R: CCAGGACACCGTAGAAAGGG |
PPARa | F: GTTGCAAGGGCTTCTTTCGG | 129 | 60°C | NM 001044526 |
R: CCGAGAGGCACTTGTGGAAA |
PTX3 | F: GCCAGCAGGTTGTGAAACAG | 121 | 60°C | NM_001244783 |
R: GGCTTTGACCCAAATGCAGG |
GJA1 | F: CTCTGAGGCGTGCCTACTTC | 77 | 60°C | NM_001244212.1 |
R: CTAAGGCACTCCAGTCACCC |
PTGS2 | F: ATCCTCCGACAGCCAAAGAC | 199 | 60°C | NM_214321 |
R: AGCCGTTCATCGTCCCATTC |
GAPDH | F: CATGGTTCACGCCCATCACA | 170 | 60°C | NM_001206359 |
R: TCCCGCCAACATCAAATGGG |