| TK F | 5’-GGGTTACCTGTACGAG-3’ | OIE (2013) | 409 |
| TK R | 5’-CACCCAGTAGATTATGC-3’ |
| SphI-5 F | 5’-GACACCACATCTGCAAGGAG-3’ | OIE (2013) | 292 |
| SphI-5 R | 5’-GACACATGTTACAATGGTCGC-3’ |
| Real time PCR |
| Primer | Sequence | Accession No. |
| IL-12p35 F | 5’-TGCTTCTCTGTCTCTGTGATGGA-3’ | AJ580354 |
| IL-12p35 R | 5’-CACAGCTGCAGTCGTTCTTGA-3’ |
| INF ɑβ F | 5’-GGCAGATATGGGACGGTGAG-3’ | EC393381 |
| INF ɑβ R | 5’-GTCCTCCACCTCAGCTTTGTC-3’ |
| TLR 9 F | 5’-GGAGACCACTCTGTTTGGAAG-3’ | GU809229 |
| TLR 9 R | 5’-CGGCATCGTTCTTGTTGTC-3’ |
| β –actin F | 5’-TCACACCACAGCCGAGAG-3’ | M24113 |
| β –actin R | 5’-CAGGGAGGAGGAGGAAGCAG-3’ |