Table 2 Specific conditions of PCRs used

Gene	Temperature & time denature / annealing / extension	Number of cycle
Cgα	94℃, 45 sec / 63℃, 30 sec / 72℃, 30 sec	35
LH-β	94℃, 45 sec / 65℃, 30 sec / 72℃, 30 sec	35
Testis LH-β	94℃, 45 sec / 65℃, 30 sec / 72℃, 30 sec	30