Table 1 Sequences of the primers used in this study

Gene	Accession number	Nucleotide sequences	Length of PCR product
Cgα	BC_063160	F 5'-ATA CTT CTC CAA GCT GGG TG	294 bp
		R 5'-CGA CAC TCA GTG CCA TCG CA
LH-β	NM_012858	F 5'-AGA TGG ACA GCC TTG TGA CC	425 bp
		R 5'-AGG ACT GCT AGC AGC ACT GT
FSH-β	BC_168724	F 5'-AAC TGC ACA GGA CAT AGC TG	344 bp
		R 5'-ACA GTG GCA TTC AGT GGC TA
KiSS-1	NM_181692	F 5'-ATC TCG CTG GCT TCT TGG CA	319 bp
		R 5'- GAC ATG TCC TTC TCG CGC TG
GnRH	NM_012767	F 5'-TCC AGC CAG CAC TGG TCC TA	170 bp
		R 5'-GCT CCT CGC AGA TCC CTA AG
GAPDH	NM_017008	F 5'-CCA TCA CCA TCT TCC AGG AG	557 bp
		R 5'-CCT GCT TCA CCA CCT TCT TG

F, forward; R, reverse.
The directions of sequences are all 5’ to 3’.