Table 1 Recalculated measurements of sperm tail motility by confocal imaging
Sperm I.D | Number of flagella motility in image (n) | The length of Y axis in sperm movement (b, µm) | Movement of sperm tail(N, number/sec) |
1 | 9 | 34.8 | 14.25 |
2 | 14 | 38.6 | 20.01 |
3 | 3 | 40.2 | 4.12 |
The measurement of the total Y lengths of the image (a) and sperm tail (b) are illustrated in Fig. 1A. The flagellar activity of the sperm cell with I.D. 1 was recalculated and shown to have normal activity. Representative results from fast-moving (I.D. 2) and slow-moving (I.D. 3) sperms are also shown. I.D., identification number.