Table 2 Comparative analysis of gonadosomatic index (GSI), sex hormone and thyroid hormone in river puffer, Takifugu obscurus, tiger puffer, T. rubripes, hybrid river puffer (♀)×tiger puffer (♂) between their parents and hybrid triploid*¹

	Initial group^*2				3 months after initial measurement^*2
	Riverpuffer	Hybrid	Hybrid triploid	Tigerpuffer	Riverpuffer	Hybrid	Hybridtriploid	Tiger puffer
Body weight (BW, g)	32.1±6.88^a	34.2±9.17^a	33.8±7.41^a	44.3±8.54^b	44.1±5.3^a	50.8±3.14^b	43.8±4.18^a	54.3±6.89^b
Viscera fat weight/BW (%)	7.7±0.54^a	10.5±1.02^b	8.9±0.77^a	11.6±1.06^b	9.7±1.81^a	13.5±1.72^b	9.5±1.81^a	13.7±1.84^b
GSI (%)^*3	0.1±0.05^a	0.1±0.02^a	0.1±0.02^a	0.4±0.02^b	0.1±0.03^a	0.1±0.04^a	0.1±0.04^a	0.4±0.03^b

*¹The values are means ± SD (n=10) of triplicate groups. Values in the same column not sharing common superscripts are significantly different among ploidy and season (P<0.05).
^*2 Allparameters of each group were measured in March to July, 2016, respectively.
^*3 Gonadosomatic index (GSI)=(Gonad weight/Body weight) ×100.
Table 3. Comparative analysis of hematological parameters in river puffer, Takifugu obscurus, tiger puffer, T. rubripes, hybrid river puffer (♀)×tiger puffer (♂) between their parents and hybrid triploid^*1