Table 3 Spermatozoa activity index (SAI) of far eastern catfish, Silurus asotus in two diluents and different concentrations of two cryoprotectants on experimental period^*

Diluent	Cryoprotectant	Concentrations(%)	SAI (Days after storage)
Diluent	Cryoprotectant	Concentrations(%)	1	180	360	540	720
Fresh sperm	-	-	2.0±0.15^a	2.1±0.13^a	2.0±0.20^a	2.2±0.19^a	2.0±0.18
no treatment	No treatment	0	0.1±0.01^a	0	0	0	0
Alsever'ssolution	No treatment	0	0.1±0.03^a	0	0	0	0
	Dimethylsulfoxide(DMSO)	5	0.4±0.07^a	0.2±0.04^a	0	0	0
		10	0.5±0.06^b	0.2±0.06^a	0	0	0
		15	0.5±0.07^a	0.3±0.03^a	0	0	0
		20	1.2±0.15^b	0.8±0.02^b	0.5±0.06^a	0.3±0.01^a	0
	Ethyleneglycol	5	0.6±0.08^a	0.4±0.05^a	0.3±0.04^a	0.2±0.04^a	0.1±0.03^a
		10	0.8±0.05^a	0.5±0.03^a	0.4±0.07^a	0.3±0.05^a	0.2±0.05^a
		15	1.5±0.19^b	1.3±0.09^b	1.0±0.08^b	0.8±0.09^b	0.6±0.09^b
		20	0.7±0.03^a	0.6±0.07^a	0.5±0.04^a	0.4±0.02^a	0.3±0.04^a
0.3Mglucose	No treatment	0	0	0	0	0	0
	Dimethylsulfoxide(DMSO)	5	0.3±0.04^a	0.1±0.02^a	0	0	0
		10	0.4±0.05^a	0.2±0.03^a	0	0	0
		15	0.4±0.08^a	0.2±0.02^a	0	0	0
		20	0.7±0.06^b	0.5±0.06^b	0.2±0.04^a	0.1±0.03^a	0
	Ethyleneglycol	5	0.5±0.02^a	0.3±0.02^a	0.1±0.01^a	0	0
		10	0.6±0.04^a	0.3±0.03^a	0.1±0.02^a	0	0
		15	1.3±0.15^b	0.9±0.10^b	0.7±0.06^b	0.5±0.05^a	0.4±0.06^a
		20	0.8±0.07^c	0.6±0.08^c	0.4±0.08^c	0.3±0.06^a	0.2±0.04^a

^*SAI: (Score × % motile sperm)/100 (After Strüssmann et al., 1994). The temperature of storage is –80℃. Mean±SD (n=100) values are shown. Means sharing the same letter superscript on given dates are not significantly different (P>0.05). Experiments are triplicate.