Table 2 Effects of lidocaine-HCl dose and starting water salinity on anesthesia among marine medaka, Oryzias dancena

Dose(mgL^-1)	Exposure time (sec)^*
Dose(mgL^-1)	0 ppt		10 ppt		20 ppt				30 ppt		40 ppt
200	250.1±14.90^a		313.3±12.43^b		323.8±38.75^c				397.4±95.78^d		396.1±63.49^d
300	182.4±21.25^a		255.8±29.20^b		255.2±33.69^b				260.9±39.18^bc		263.0±30.29^c
400	150.0±33.78^a		170.1±17.76^b		187.9±15.38^c				200.9±27.14^d		199.0±16.46^d
500	110.4±15.46^b		105.3±32.22^a		158.9±28.97^c				167.1±23.27^c		167.9±28.31^c
600	83.0±15.32^a		82.4±28.19^a		134.9±27.12^b				134.8±16.75^b		138.8±43.29^c
700	76.0±16.63^b		62.4±15.66^a		95.6±35.76^c				99.5±0 9.14^c		101.5±34.31^d
800	57.0±8.91^b		47.6±7.21^a		74.7±12.05^c				76.6±0 4.86^c		78.7±17.18^c
Dose(mgL^-1)	Recovery time (sec)^*
Dose(mgL^-1)	0 ppt		10 ppt			20 ppt		30 ppt			40 ppt
200	379.0±35.45^d		208.2±20.83 ^a			330.0±37.33^c		310.4±39.40^b			308.2±58.47^b
300	350.0±24.81^d		234.4±131.83^a			320.5±73.06^c		305.2±34.50^b			300.5±40.80^b
400	327.2±68.13^d		260.5±169.29^a			280.5±48.56^c		272.2±38.34^b			270.4±84.46^b
500	325.4±68.10^d		214.0±92.94^a			238.5±38.07^c		223.8±35.34^b			220.1±53.95^b
600	325.8±156.61^b		168.7±179.91^a			169.8±29.72^a		171.1±20.71^a			170.0±33.56^a
700	323.3±67.40^c		146.9±56.28^a			167.0±43.54^b		166.8±31.70^b			166.9±40.39^b
800	322.1±60.25^c		129.6±70.31^a			132.3±20.15^b		133.1±15.88^b			132.5±24.74^b
Two-way ANOVA (exposure time)
	DF	Anova SS		Mean square			F-value			P-value
Salinity	4	179,621.5		4,905.379			46.625			<0.0001
Dose	6	2,583,927		430,654.5			447.147			<0.0001
Interaction	24	92,106.646		3,837.777			3.985			<0.0001
Two-way ANOVA (recovery time)
	DF	Anova SS		Mean square			F-value			P-value
Salinity	4	638,164.1		159,541.0			52.927			<0.0001
Dose	6	1,248,225		208,037.6			69.016			<0.0001
Interaction	24	191,254.8		7,968.949			2.644			<0.0001

^* Each value is mean±standard error of duplicate experiments. Values in the same column not sharing common superscripts are significantly different (P<0.05).