Table 1 Effects of clove oil dose and starting water salinity on anesthesia among marine medaka, Oryzias dancena

Dose(mgL^-1)	Exposure time (sec)^*
Dose(mgL^-1)	0 ppt		10 ppt		20 ppt	30 ppt			40 ppt
50	160.0±9.43^b		126.6±11.02^a		206.0±19.83^c	218.6±40.25^d			226.4±39.10^e
75	138.4±8.28^b		83.0±18.38^a		154.7±16.46^c	159.9±39.76^d			164.3±17.24^e
100	100.8±4.29^b		61.5±5.52^a		108.9±6.31^c	112.2 ±15.75^d			120.4±7.88^e
125	73.5±12.61^b		52.1±4.91^a		80.9±11.59^c	85.2±6.61^d			90.9±8.97^e
150	50.1±6.06^a		44.2±2.94^a		62.9±4.93^b	62.8±8.93^b			73.8±9.77^c
Dose(mgL^-1)	Recovery time (sec)^*
Dose(mgL^-1)	0 ppt		10 ppt		20 ppt	30 ppt			40 ppt
50	185.0±26.94^c		133.2±14.81^a		163.2±26.33^b	160.8±20.84^b			159.1±21.48^b
75	188.5±29.02^d		125.7±18.49^a		155.7±15.10^b	145.9±16.04^c			145.8±18.59^c
100	196.5±22.98^c		116.7±13.28^a		146.7±21.73^b	145.6±20.58^b			146.2±20.05^b
125	193.8±34.00^d		123.5±20.38^a		143.5±20.97^b	151.9±13.03^bc			152.1±12.04^c
150	175.6±33.94^d		131.3±9.20^a		157.4±12.10^b	151.3±14.33^c			157.5±13.20^b
Two-way ANOVA (exposure time)
	DF	Anova SS		Mean square			F-value	P-value
Salinity	4	30,475.440		7,618.860			26.055	<0.0001
Dose	4	618,919.0		154,729.7			529.150	<0.0001
Interaction	16	12,833.400		802.087			2.743	<0.0001
Two-way ANOVA (recovery time)
	DF	Anova SS		Mean square			F-value	P-value
Salinity	4	48,654.896		12,163.724			29.521	<0.0001
Dose	4	5,557.856		1,389.464			3.372	<0.0110
Interaction	16	9,551.864		596.992			1.449	<0.1210

^*Each value is mean±standard error of duplicate experiments. Values in the same column not sharing common superscripts are significantly different (P<0.05).