Table 3 The number of unique loci to each species and number of shared loci by the three species generated by PCR research using 7 oligonucleotides primers in Styela clava (SC), Halocynthia roretzi (HR) and Styela plicata (SP) species, respectively

Item	No. of unique loci to each species			No. of shared loci by the three species
Primer \ Species	SC	HR	SP	Three species (7 individuals per species)
BION - 11	21	21	21	0
BION - 13	21	7	0	0
BION - 17	28	7	7	21
BION - 21	14	0	7	0
BION - 23	21	14	28	21
BION - 25	14	0	7	0
BION - 30	7	0	0	0
Total no.	126	49	70	42
Average no. per primer	18	7	10	6

SC: Styela clava, HR: Halocynthia roretzi, SP: Styela plicata