Table 2 Primers used for quantitative real-time PCR to investigate the expression of P450aromA, P450aromB, estrogen receptor alpha, GnRH1, GnRH receptor 1, FSHβ, LHβ and common glycoprotein α

Genes(Accession number)		Primer sequence	Product length
Aromatase-a(AY547354)	Forward	5’- ACGCCATAGAAAGTCTTGTAG - 3’	79
	Reverse	5’- GCAGTGAAGTTGATGTTATCG - 3’
Aromatase-b(AY547353.1)	Forward	5’- GGAGGTCCTTCTGGAATGTAAAC - 3’	102
	Reverse	5’- CCGACGTTCTGAGCAAAGATAA - 3’
	Reverse	5’- GCCCATTTGACCACTTTGAC - 3’
Estrogen receptor alpha	Forward	5’- CAACTCTGGTGCCTTCTCTTT - 3’	119
	Reverse	5’- CCGACTGGCTGATATGATGTATG - 3’
GnRH1	Forward	5’- GACACACCGGGCAATATTCT - 3’	79
	Reverse	5’- GTGATTCCTCCACACAACCA - 3’
GnRH-receptor1	Forward	5’- TGTGCTGGACTCCGTACTA - 3’	110
	Reverse	5’- CAGGTTCCCAAACAGGAAGA - 3’
FSHβ(KJ534537.1)	Forward	5’- GACTGGACCTATGAGGTGAAAC - 3’	147
	Reverse	5’- AAAGGACAGACAGCTGGATATG
LHβ(KJ534538.1)	Forward	5’- GGGAGCCTCATCTTCCATTT - 3’ - 3’	108
	Reverse	5’- TTTGGACAGCCTTCCTTCTC - 3’
Common glycoprotein α(AY207430.1)	Forward	5’- CGATCCCGAAGAACATCATCTC	96
	Reverse	5’- CTGTGTGGTTTCTCACCCTTAT - 3’ - 3’
β-actin(HQ007251)	Forward	5’- GACCTCACAGACTACCTCAT - 3’	96
	Reverse	5’- GCTTCTCCTTGATGTCACG - 3’