| Gene(GenBank ID) | Primer sequence(5’ → 3’) | Tm(℃) | PCR productsize (bps) |
| Cx26(NM_001004099) | (F) TCCTCTTCATCTTCCGCATC(R) CCGTTTCTTTTCGTGTCTCC | 55 | 233 |
| Cx30.3(NM_053984) | (F) CCCAATGTCTGCTATGACGA(R) CACAGCAGCCTTGAAGATGA | 57 | 243 |
| Cx31(NM_019240) | (F) TTGAGCGGTGTGAACCAGTA(R) TGTTGGAGATGGGGAAGAAG | 57 | 193 |
| Cx31.1(NM_019241) | (F) CATCGTCTGCATCCTGCTTA(R) ATGAGGTCGCTTGAGAGGAA | 55 | 165 |
| Cx32(NM_017251) | (F) AGAATCATGGTGCTGGTGGT(R) CCTCAAGCCGTAGCATTTTC | 57 | 235 |
| Cx37(NM_021654) | (F) AGTGTCTGTACCTTGGATGCC(R) CAGCACACTTAGCCAAGAGC | 51 | 223 |
| Cx40(NM_019280) | (F) ATACCATTCAGCCTGGTTGC(R) CGGCCTCTTTAGCTTTCTCA | 57 | 189 |
| Cx43(NM_012567) | (F) AGCAAGCTAGCGAGCAAAAC(R) GAGTTCATGTCCAGCAGCAA | 55 | 151 |
| Cx45(NM_001085381) | (F) GATCATCCTGGTTGCTACTC(R) GATCCTCTTCATGGTCCTCT | 51 | 173 |
| Ppia(NM_07101.1) | (F) GGCAAATGCTGGACCAAACAC(R) TTAGAGTTGTCCACAGTCGGAGATG | 59 | 196 |