Table 1 Oligonucleotide primers utilized for real-time PCR analysis

Gene (GenBank ID)	Primer sequence (5’ → 3’)	Tm (°C)	PCR product size (bps)
Cx26	(F) TCCTCTTCATCTTCCGCATC	55	233
(NM_001004099)	(R) CCGTTTCTTTTCGTGTCTCC
Cx30.3	(F) CCCAATGTCTGCTATGACGA	57	243
(NM_053984)	(R) CACAGCAGCCTTGAAGATGA
Cx31	(F) TTGAGCGGTGTGAACCAGTA	57	193
(NM_019240)	(R) TGTTGGAGATGGGGAAGAAG
Cx31.1	(F) CATCGTCTGCATCCTGCTTA	55	165
(NM_019241)	(R) ATGAGGTCGCTTGAGAGGAA
Cx32	(F) AGAATCATGGTGCTGGTGGT	57	235
(NM_017251)	(R) CCTCAAGCCGTAGCATTTTC
Cx37	(F) AGTGTCTGTACCTTGGATGCC	51	223
(NM_021654)	(R) CAGCACACTTAGCCAAGAGC
Cx40	(F) ATACCATTCAGCCTGGTTGC	57	189
(NM_019280)	(R) CGGCCTCTTTAGCTTTCTCA
Cx43	(F) AGCAAGCTAGCGAGCAAAAC	55	151
(NM_012567)	(R) GAGTTCATGTCCAGCAGCAA
Cx45	(F) GATCATCCTGGTTGCTACTC	51	173
(NM_001085381)	(R) GATCCTCTTCATGGTCCTCT
Ppia	(F) GGCAAATGCTGGACCAAACAC	59	196
(NM_07101.1)	(R) TTAGAGTTGTCCACAGTCGGAGATG

Cx : connexin; Ppia : peptidylprolyl isomerase A (cyclophilin A).