Table 2 Specific conditions of PCRs used in this study

Gene	Temperature & Time Denature / Annealing / Extension	Number of cycle
StAR	94°C, 40 sec / 61°C, 1 min / 72°C, 1 min	35
CYP11A1	94°C, 30 sec / 65°C, 40 sec / 72°C, 40 sec	35
CYP17A1	94°C, 40 sec / 64°C, 1 min / 72°C, 1 min	33
3β-HSD	94°C, 30 sec / 61°C, 40 sec / 72°C, 40 sec	38
17β-HSD	94°C, 30 sec / 61°C, 40 sec / 72°C, 40 sec	35
CYP19	94°C, 30 sec / 60°C, 40 sec / 72°C, 40 sec	33
LH-R	94°C, 30 sec / 61°C, 40 sec / 72°C, 40 sec	32
GAPDH	94°C, 30 sec / 61°C, 40 sec / 72°C, 40 sec	24