Table 1 Primer sequence of undifferentiation markers of hESCs

Marker	Primer sequence
GAPDH	F: AGCCACATCGCTCAGACACC R: GTACTCAGCGGCCAGCATCG
Oct-4	F: CTACAACGCCTACGAGTCCTACA R: TTCTGGCGCCGGTTACAGAACCA
Nanog	F: CTGAGATGCCTCACACGGAGACTG R: GTCACACCATTGCTATTCTTC
Rex1	F: GCGTACGCAAATTAAAGTCCAGA R: CAGCATCCTAAACAGCTCGCAGAAT
Sox2	F: GGCAGCTACAGCATGATGCAG R: GCTCTGGTAGTGCTGGGACATG