Table 1. Changes in spermatocrit, sperm density, and milt production in the mature grass puffer, Takifugu niphobles, after an injection of human chorionic gonadotropin (HCG)*

Exp. group (IU/kg BW)	Hours after injection	Spermatocrit (%)	Sperm density (×105 spz/μL milt)	Milt production (mL/kg BW)
Cont.	0	75.0 ± 0.79Aa	8.0 ± 0.85Aa	57.8 ± 17.19Aa
500		75.2 ± 0.57Aa	7.9 ± 1.24Aa	53.4 ± 13.24Ab
1,000		75.6 ± 0.96Aa	7.8 ± 1.39Aa	52.9 ± 14.29Bb
2,000		75.1 ± 0.74Aa	8.1 ± 1.13Aa	55.6 ± 15.34Ab
Cont.	24	72.0 ± 9.85Aa	6.5 ± 0.30Ab	57.6 ± 12.94Ca
500		65.0 ± 14.11Bb	7.5 ± 1.19Ba	22.4 ± 33.81Dc
1,000		50.3 ± 5.03Cb	7.2 ± 0.92Bb	109.4 ± 34.76Aa
2,000		44.0 ± 6.93Db	5.4 ± 0.71Cb	96.0 ± 15.24Ba
Cont.	48	64.7 ± 11.93Ab	6.7 ± 0.67Ab	17.1 ± 9.53Cb
500		61.0 ± 5.57Ab	6.6 ± 2.45Bb	58.3 ± 27.07Aa
1,000		54.7 ± 7.02Bb	6.6 ± 2.82Bc	41.8 ± 9.32Bc
2,000		40.7 ± 7.23Cb	5.4 ± 1.69Cb	42.1 ± 4.62Bc

Means ± SD (n = 50) of triplicate groups. Data were analyzed using one- and two-way ANOVA.
Different capital letters on the values indicate statistically significant differences among doses at each time after injection (P < 0.05).
Different small letters on the values indicate statistically significant differences among times after injection for each dose (P < 0.05). Cont.: control group; Exp.: experimental group; spz.: spermatozoa.