Table 1.
The survival ratio of embryos in vitro culture with different FBS concentration

Embryonic	No. of survival
Culture medium	Blastodisc	Early-eyed	Very late-eyed	Larvae
L-15(5% FBS)	30	0
L-15(10% FBS)	30	19	9	5
L-15(15% FBS)	30	17	7	3
L-15(20% FBS)	30	13	0

No heartbeating or body-fluid exchanging embryos were considered as dead.