Table 1.
Sequences of primers, RT-PCR conditions, and their expected product sizes

Gene	Accession no.	Primer sequence*	Annealing temperature (°C)	Product size (bp)
Ptx3	NM_008987.3	F : 5’-GCATCCTGTGAGACCAATGA-3’R : 5’-GGGTTCCACTTTGTGCCATA-3’	60	111
Has2	NM_008216.3	F : 5’-GCTGTACAATGCCATGTGGT-3’R : 5’-GGATGACTGTGGCAATGAGA-3’	60	101
Tnfaip6	NM_009398.2	F : 5’-TCCACGGCTTTGTAGGAAGA-3’R : 5’-GACGGATGCATCACTCAGAA-3’	60	101
Gja1	NM_010288.3	F : 5’- AAGAACACGGCAAGGTGAAG-3’R : 5’- TACCACTGGATCAGCAGGAA-3’	60	106
Gja3	NM_001271623.1NM_016975.3	F : 5’-TCATCTTCATGCTGGCTGTG-3’R : 5’-GCATCTGGGTTGAAGTGGTT-3’	60	115
Gja4	NM_008120.3	F : 5’-CAGCAACCTGAGCAAGAAGA-3’R : 5’-CACAGCAGCCTTGAAGATGA-3’	60	73
Gja10	NM_010289.2	F : 5’-TGCAAGAGAGAGTTGGGGTTT-3’R : 5’-CACTATGCCGTTGTCCCTTT-3’	60	101
Gjb1	NM_008124.2	F : 5’-AGAGACACAAGGTGCACATCTC-3’R : 5’-CATGAAGACAGCCTCGAACA-3’	60	89
Gjc1	NM_001159382.1NM_001159383.1NM_008122.3	F : 5’-TGTGTGCAGCAGACTTCCTT-3’R : 5’-CCCAAACCCTAAGTGAAGCA-3’	60	148
FSHr	NM_013523.3	F : 5’-TGTGGTCATCTGTGGTTGCT-3’R : 5’-GCTTGGCAATCTTGGTGTCT-3’	60	92
LHr	NM_013582.2	F : 5’-AGTGGCCTTTGTCGTCATCT-3’R : 5’-GCCATGCATGTGAAGTCTGT-3’	60	141
Gdf9	NM_008110.2	F : 5’-TTGGCAGTCTCTTCAGTCCA-3’R : 5’-GGGAGATCTTTCCACCTCAA-3’	60	106
Prps1	NM_021463.4	F : 5’-TTGATATCCCGGTGGACAAT-3’R : 5’-AGGGCCAGAAAAGATTCCAT-3’	60	356
Rpe	NM_025683.2	F : 5’-CTTGCCATCAAACCAGGAAC-3’R : 5’-GCACTGCCAGACACAATCAT-3’	60	248
Rpia	NM_009075.2	F : 5’-TGCAGCGAATAGCTGAAAGA-3’R : 5’-ACAGCCATTCGAAGTTCCAC-3’	60	394
Taldo1	NM_011528.4	F : 5’-TGACGCTCATCTCTCCCTTT-3’R : 5’-GCCAGCTTGCTGTTATCCTT-3’	60	365
Tkt	NM_009388.5	F : 5’-TCGGAGCTCTTCAAAAAGGA-3’R : 5’-TCTCTGTTGCAACTCCATCG-3’	60	325
H1foo	NM_008872	F: 5’-GCGAAACCGAAAGAGGTCAGAA-3’R: 5’-TGGAGGAGGTCTTGGGAAGTAA-3’	60	378
Gapdh	BC092294	F: 5’-ACCACAGTCCATGCCATCAC-3’R: 5’-TCCACCACCCTGTTGCTGTA-3’	60	451

F, forward primer; R, reverse primer.