Table 2.
Sequences of primers used for expression studies

		Sequence	Product size (bp)
Tissue distribution (RT-PCR)	mel 1a F	5'-ACG CAG GGA ACA TCT TTG TG-3'	318
	mel 1a R	5'-CAC AAA CAG GTT GGG CAC GAT-3'
	mel 1b F	5'-TCG CCC AGA ATG TCA GCA GT-3'	221
	mel 1b R	5'-GGC CCA GCA AAT GGC AAA CA-3'
	mel 1c F	5'-TAC CCC TAC CCA CTG GTC CT-3'	234
	mel 1c R	5'-TGG CGG TGA GTA GCC AAG TA-3'
	β-actin F	5'-AAT CGT GCG TGA CAT CAA GG-3'	392
	β-actin R	5'-AGT ATT TAC GCT CAG GTG GG-3'
	Quantitative real-time PCR	qmel 1a F	5'-GGT TGT GAT TCC CCT CAT CCC-3'	105
		qmel 1a R	5'-TTC TGG TTC AGC ACG CCA TAC A-3'
		GnRHr I F *	5'-GTG GCT TGC CGG AGA CTT TG-3'	123
GnRHr I R *		5'-AGA GGG TTG AGG ATG GCT GAC T-3'
		qβ-actin F	5'-AGC ATC CCG TCC TGC TCA CA-3'	121
		qβ-actin R	5'-AGC ACA GCC TGG ATG GCA AC-3'

GnRHr I gene primer from Martinez-Chavez et al. (2008). Primers for mel 1a, mel 1b (GenBank accession No. XM003446809.1), mel 1c (Kim et al., unpublished) and β-actin (GenBank accession No. XM003443127.1) genes were newly designed.